MINERALS

 

MINERALS

  Outcomes:

At the end of this unit, the learner should be able to:

·       List the major minerals and describe their physiologic functions in the body.

·       List the dietary sources of each of the minerals.

·       Outline the factors affecting the absorption and utilization of the minerals.

·       Identify the factors that influence the bioavailability of the minerals from food.

·       Describe the relationship between minerals and other nutrients in the body.

·       Describe the health problems caused by mineral deficiencies, their signs and symptoms and there prevention.

 

In the previous topic we discussed one of the major micronutrients, vitamins. In this topic we will review the remaining micronutrient, minerals. Like vitamins, they perform important functions in the body. They account for 4% of body weight. They are found in all body fluids and tissues. Unlike the energy nutrients and vitamins, minerals are inorganic elements that originate from the earth’s crust, not from plants and animals. Minerals do not undergo digestion nor are they broken down or rearranged during metabolism. Although they combine with other elements to form salts (e.g. sodium chloride) or with organic compounds (e.g. iron in haemoglobin) they always retain their chemical identities. Unlike vitamins, minerals are not destroyed by light, air, heat or acid during food preparation. In fact when food is completely burned, minerals are the ash that remains. Minerals are lost only when foods are lost in water.

Definition of terms

·       Major minerals:

These are essential mineral nutrients required in the adult diet in amounts greater than 100 milligrams per day.

·       Trace minerals:

Essential mineral nutrients required in the adult diet in amounts less than 100 milligrams per day.


 

·       Fluid and electrolyte balance:

Maintenance of the necessary amounts and types of fluid and minerals in each compartment of the body fluids.

·       Acids: compounds that release hydrogen ions in solution.

·       Bases: compounds that accept hydrogen ions in solution.

·       Acid- base balance: The balance maintained between acid and bases concentrations in the blood and body fluids.

·       pH: The concentration of hydrogen ions. The lower the pH, the stronger the acid. Thus pH 2 is a strong acid while pH 6 is a weak acid; Ph 7 is neutral; and a pH above 7 is alkaline.

·       Buffers: Compounds that can reversibly combine with hydrogen ions to help keep solutions acidity constant.

·       Ossification: The process of bone formation.

·       Osteomalacia: A weakening of the bones due to a calcium, phosphorus, and/or Vitamin D deficiency.

·       Osteoporosis: An abnormal porousness of bone as the result of a calcium, phosphorus, and/or vitamin D deficiency.

 Classification

Minerals are classified into two groups based in the amount found in the body. Some occur in large quantities and are required in quantities above 100mgs per day. These are referred to as macro-minerals and they include calcium, phosphorus, potassium, sodium, chloride, magnesium and sulfur.

Others occur in small amounts and are required in only a few milligrams (15mgs/day or less). They are therefore considered as trace elements or micro-minerals and they include iron, copper, cobalt, zinc, manganese, iodine, molybdenum, fluorine, selenium and chromium.

6.5General Functions of Minerals include:

Minerals perform several general functions because of their interrelationship. They provide structure to the body tissues and regulate body processes such as fluid balance, acid-balance, nerve transmission, muscle contraction, vitamin, enzyme and hormonal activities.

a.   Structure

Calcium, phosphorus, magnesium and fluoride provide structure to bones and teeth. Soft tissues gain structural support from phosphorus, potassium, iron and sulphur. Sulphur is also a fundamental constituent of skin, hair and nails.

b.   Maintenance of acid-based balance

This term refers to the maintenance of the body’s concentration of hydrogen ions (pH). Biological reactions in the cell occur when the pH is right. Anything that changes the pH of the cell environment may change the way cellular enzymes act.  Inactivation of enzymes results in starvation and death of cells. Acid forming minerals such as, chlorine, sulphur and phosphorus are found in the extra cellular fluid.  They predominate in foods rich in protein such as meat, fish, poultry, eggs and cereal products (acid forming foods). Basic or alkaline minerals such as calcium, sodium, potassium and magnesium tend to predominate in fruits, vegetables and nuts.  Milk contains internal balance of base forming calcium and acid forming phosphorus, and does not influence acid-base balance

c.   Catalysts for biological reactions

Minerals serve as co-factors for many biological reactions. They are not part of initial compounds or end products but they serve as vital links between enzymes and the substance which they act on e.g. zinc in known to catalyze about 100 different reactions. They catalyse many of the separate steps involved in the metabolism of carbohydrates, fats and proteins. For example, the synthesis of haemoglobin depends on several mineral elements that are not part of haemoglobin such as iron. Similarly, although calcium is a catalyst for blood clotting, it is not part of the clot. The absorption of nutrients from the gastrointestinal tract and the uptake of nutrients by the cells often depend on minerals (e.g. calcium facilitates the absorption of B12, sodium and magnesium facilitates absorption of carbohydrates).

d.   Components of essential body compounds

Many hormones, enzymes and other vital compounds synthesized in the body contain minerals as essential parts of their structure. As such, if the required mineral is absent, the body will be unable to produce adequate amounts of these substances. For example, thyroxin, a hormone which controls energy metabolism requires iodine.  Haemoglobin which transports oxygen to the cells and carbon dioxide from them contains iron; chlorine is essential for hydrochloric acid production in the stomach.  Mineral containing enzymes are sometimes called metalloenzymes. If the mineral is unavailable, the enzymes will not be synthesized or will be ineffective.  Molybdenum is part of an enzyme needed to release liver stores of iron. Zinc is part of a protein splitting enzyme carboxypeptidases secreted into the pancreatic juice.

e.   Maintenance of fluid balance

Water comprise of 60% total body weight or 70% of fat free weight. It is present in 3 components, separated by semi-permeable membrane that allows free exchange of fluids and electrolytes (ions). Intravascular (in blood) compartment includes fluid in the arteries, veins and capillaries. Intercellular (between cells) bathes individual cells and provides nourishment, this act as a buffer area. Its volume will adjust to prevent changes in the volume of the intravascular or extra cellular chambers. Intracellular fluid is found within the cells. Transcellular compartment includes the fluid which lubricates the joints and fluid which in the eyeball. This represents a very small portion and it is not involved in fluid shifts.

The movement of fluid from one compartment to another is controlled by the concentration of minerals on either side of the membranes. Mineral elements in body fluids occur as salts that separate into component ions with positive and negative charges. Charged ions are known as electrolytes and maintain osmotic pressure of body fluids. As the concentration of electrolytes increase, osmotic pressure increases, this draws fluid from the compartment with low electrolyte concentration until the concentration (or pressure) is equalized. Under normal circumstances, the body is able to prevent a shift in electrolyte concentration between compartments. Shifts however do occur in abnormal situations.

f.    Transmission of nerve impulses

Minerals play a vital role in conducting nerve impulses along a nerve fibre. The exchange of sodium and potassium across nerve causes the transmission of nerve impulses. The permeability of membrane changes allowing potassium to leave the cell. At the same time sodium enters each cell, but is quickly pumped out, this creates a temporary change in the electrical charge. In this way the message is passed along the fibre. The transmission of a nerve impulse from one nerve cell to another is also dependent on neurotransmitter acetylcholine (ACH) at the junction of the two fibres. The release of ACH is regulated by calcium.

g.    Regulation of muscle contraction:

The muscles of the body are bathed in the intercellular fluid. For muscles to function normally, the composition of this fluid should have a balance between mineral elements such as calcium which stimulates muscular contraction and sodium, potassium, and magnesium which exerts relaxing effect. This balance does not depend on dietary sources and can only be disturbed by hormonal changes.

h.   Growth of body tissue

Some minerals such as calcium and phosphorus occur in large concentration in bones and teeth and are the building material of body tissue. Absence of these will lead to stunted growth or poorly developed bones and teeth. Other minerals serve as catalysts to reactions which lead to synthesis of body compounds or to release energy needed for growth.

Macro Minerals

6.4.1Calcium

Most abundant mineral present in the body, amounting to almost 40% of the total mineral mass.

Majority is present in bone, where together with phosphorous, it plays an essential part in hardening the skeleton and teeth.

In addition, calcium is a reserve of the mineral for its role in body fluids as ionic calcium, which is essential for nerve impulse transmission, muscle contraction and blood clotting.

a.   Absorption of Calcium

Calcium salts are generally not highly soluble, which makes their absorption from the diet problematic.  Several factors can enhance or inhibit absorption of Calcium.

Factors enhancing calcium absorption

1.   Vitamin D, which causes the synthesis of a calcium-binding protein in the intestinal cells that, transports calcium into the plasma.

2.   Lactose (present in milk) also enhances calcium absorption by keeping it in a soluble form.  The presence of lactose and the large amounts of calcium found in milk are make this an excellent source of the mineral.

3.   Other sugars and protein also enhance calcium absorption.

4.   The acidic environment of the upper digestive tract also facilitates the solubility of calcium.

5.   Body Need - This is the major factor governing the amount of calcium that will be absorbed during periods of growth, low calcium levels in the body fluids, and when body stores are depleted, more calcium is absorbed.

6.   Other factors that enhance absorption are;

-        The acidic environment in the upper gastro intestinal tract

-        The normal gastro intestinal motility


 

Factors inhibiting calcium absorption

·       Calcium absorption is reduced by phytic acid present in whole cereals, owing to the formation of insoluble calcium phytate.

·       Oxalates (present in spinach, rhubarb, beetroot, chocolate, tea infusions, wheat bran, peanuts and strawberries) may also inhibit calcium absorption owing to the insoluble nature of the calcium oxalate salt

·       Non-starch polysaccharides may trap some calcium making it unabsorbable in the small intestine.  However fermentation of the soluble NSP in the large intestine may release the calcium for absorption here.

·       Unabsorbed fats will combine with calcium to form soaps, removing calcium from the body.  This is a particular problem in steatorrhea, in which loss of vitamin D, a fat-soluble vitamin, may aggravate the problem of calcium absorption.

The skeleton is an active reservoir of calcium.

Role of calcium in the body

In bones – Calcium is principally located in the bones.  The mineral is continually laid down by osteoblasts and resorbed by osteoclasts.  Bone growth happens in childhood and adolescence and maintained in adults.  Changes in levels of some hormones such as cortisol or oestrogens will cause bone loss.   The amount of bone declines with age.  It happens earlier and rapidly in women at the time of menopause. When bone breakdown exceeds repair the bones become fragile and can easily break.  This condition is known as osteoporosis.

In blood and body fluids – Necessary for homeostasis where levels are maintained at 2.2 – 2.6 mmol/L.  When plasma calcium is low (or phosphate levels are high) parathyroid hormone is secreted.  This increases calcium level by promoting synthesis of active vitamin D and thus, increasing calcium absorption from the gut, reducing calcium excretion at the kidney and stimulating calcium release from the bone.  Conversely, high calcium levels cause the release of calcitonin from the thyroid gland.  This inhibits bone mobilization and promotes calcium uptake into bone.

Calcium is essential for blood clotting where insoluble prothrombin is converted to thrombin by the action of fibrin and other clotting factors.  If calcium levels are insufficient, blood will not clot.

Muscle contraction and nerve impulse contraction at nerve endings both involve the movement of calcium across the cell membrane, increasing intracellular levels and triggering contraction or depolarization.

Calcium is required for the normal transmission of nerve impulses. The calcium ions operate at the tips of nerve branches and help to excite the muscle fibre.

Calcium activates a number of enzymes, including pancreatic lipase (digest fat) and adenosinetriphosphate (involved in the release of energy for muscular activity). Calcium also aids in the absorption and utilization of vitamin B12.

Permeability of the cell membrane - Calcium regulates the passage of substances across cell membranes

Calcium excretion

Calcium is lost from the body via urine and faeces, with very small amounts through sweat.

Urinary calcium represents the final adjustment of calcium levels, with the majority (up to 97%) of the calcium filtered being reabsorbed by the renal tubules.

Levels of urinary calcium are increased:

·       On a high calcium intake

·       By a high protein diet

·       By high sodium intake

·       In women at the menopause

·       In old age

·       By a high potassium intake

·       By a high magnesium intake

·       By a high phosphorous intake

Factors that help to develop peak bone mass

·       Body weight – Excessively thin females with BMI below 20 may be amenorrhoeic.  The absence of normal menstrual cycles and lack of oestrogen will prevent normal bone accretion.  This may be a problem in girls with eating disorders.  Body size is a good indicator of bone mass.  Muscular development increases bone development to support movement.

·       Alcohol and smoking.  These both reduce bone accretion and are therefore detrimental to bone health

·       Vitamin D.  Adequate exposure to sunlight is important for vitamin D synthesis which is necessary for calcium absorption.

·       Vitamin K is important to facilitate bone development (Osteocalcin and matrix glaprotein are dependent on Vitamin K).

·       Vitamin C.  This is an essential factor in the synthesis of collagen that forms part of the structural framework of bones.

Maintenance of Serum Calcium Levels

Serum calcium levels and the ratio of serum-calcium to phosphorous are held relatively constant at the expense of bones if dietary intake of calcium is not adequate. To maintain blood calcium concentration within normal limits, several hormones play an interactive role, whereby they control the amount of calcium absorbed and excreted, and also bone metabolism. The hormones include active vitamin D, PTH, calcitonin, estrogen, testosterone and other. They respond to changes in the serum calcium levels in three ways.

Active Vitamin D increases calcium absorption, mobilizes calcium from the bones and increased renal absorption to some extent

PTH also increases calcium absorption, mobilizes calcium from the bones, and increases renal re-absorption.

Calcification helps to correct high levels by preventing further bone re-absorption.

Note:

Altered serum calcium levels are usually due to hormonal abnormalities or may occur secondary to other clinical conditions such as inadequate secretion of PTH due to hypoparathyrodism which may be surgically induced or otherwise (e.g. in thyrodectomy, or neck dissection)

Hypercalcemia can cause nausea, vomiting anorexia, abdominal pain, constipation, polydipsia, mental changes, calcium renal stones and excessive calcification of bones and soft tissues. Coma and death may result if not treated.

Hypercalcemia may be cause by cancer excessive combined intakes of calcium and Vitamin D, hyperparathyroidism (increase secretion of parathyroid hormone) thyroid disorders and hypophosphatemia.

Re-absorptive Hypercalciuria is a condition that occurs during periods of prolonged immobility, when an excessive amount of calcium is withdrawn from the bones and excreted in urine, thus increasing the risk of calcium renal stone formation.


 

Deficiency

Calcium deficiency may be related to an inadequate intake or may occur secondary to mal-absorption syndromes, vitamin D deficiency or endocrine disorders. A long standing deficiency will lead to:

·       Rickets and badly formed teething children

·       Hypertension

·       Osteoporosis is adults due to withdrawal of calcium from the bones to raise the serum calcium levels, leading (bone anthrop) and resulting in shortening of stature. The bones are also porous and brittle and fracture very easily. 

Note: Exercises appear to reduce loss of calcium from bones; also there is clear evidence that providing the female hormone estrogen to women after menopause reduces bone loss and osteoporosis.

·       Stunted growth in children

·       Tetany – a condition characterized by uncontrolled muscular contractions (spasms) and failure to relax thus causing the body to stiffen with pain, due to low serum calcium levels or too much phosphorous, which causes a decrease in calcium absorption.

·       Failure of blood clotting.

Food sources

All the calcium in the human body except that inherited from the mother, comes from food and water consumed. It is necessary to have adequate quantities of calcium she draws extra supplies from her bones. An entirely breastfed infant will obtain adequate calcium from breast milk as long as the volume of milk is sufficient.

A lactating mother secreting 1 litre will thus lose 300mg of calcium per day.

Cow’s milk is a very rich source of calcium (richer than human milk). A litre of human milk contains 300mg of calcium than a child needs hence it is meant for the calf.

Other rich sources

Excellent Sources: milk, hard cheese, yoghurt, dark green leafy vegetables and sardines.

Good Sources: Softer cheese, ice cream and dried legumes

Fair Sources: oranges, nuts and eggs

Poor sources: most of other fruits, vegetables and grains and meats.


 

Calcium Content of Various Milks

Sources of Milk           Calcium Content (mg/10ml)

Human                                      32

Cow                                           119

Camel                                                  120

Goat                                          134

Water Buffalo                                      169

Sheep                                        193

The calcium content of drinking water varies from place to place. Hard water usually contains high levels of calcium.

Recommended dietary requirements

It is not easy to state categorically the human requirements for calcium, because there are several factors influencing absorption and considerable variations in calcium losses among individuals. Need for calcium is increase during pregnancy and children require more calcium because of growth. Those on high protein diets require more calcium in the diet. The following are recommended levels of daily calcium intake:

Children ages 1 to 10 years                         800mgs/day

For men and women aged 18 to 24 years    1200mgs/day

Adults aged 25 years and above                  800mgs/day

Pregnant and lactating mothers’                  1200mgs/day

 

Phosphorus

After calcium, the most abundant mineral in the body is phosphorus. It constitutes 22% of the minerals in the adult human body or 1% of the body weight. It is a major constituent of bones and teeth or 85-90% of total body’s phosphorus. The remainder is distributed in body tissues with half in muscles. It is part of the nucleic acids; DNA and RNA which are found in the nucleus of every living cell. As a phospholipid, it is a structural component of the cell wall and participates in all biological reactions.

 


 

 Functions of phosphorus

1.   Regulation of energy release from oxidation of carbohydrates, fats and proteins: Energy is stored as ATP (adenosine triphosphate) and when energy in needed, ATP is changed to ADP (adenosine diphosphate).

2.   Absorption and transport of nutrients: Phosphorus in the form of phosphate makes it possible for nutrients to cross cell membranes or be carried in the blood. This process is known as phosphorylation and occurs in several areas: absorption in the intestines, release of nutrients from the blood stream to the intercellular fluids, uptake into the cells, and uptake by organelles of the cell. When glycogen is released from the liver or muscles for energy, it appears in the blood combined with phosphorus.

3.   Part of essential body compounds: Some vitamins and enzymes can function only when they are phosphorilated e.g. thiamin pyrophosphate.

4.   Essential part of DNA and RNA which are essential for cell reproduction.

5.   Calcification of bones and teeth.

6.   Regulation of acids-base balance: Serves as buffers since they can take additional hydrogen ions.

Absorption and metabolism of phosphorus

It is released from food by enzymes known as phosphatases. It is absorbed with the help of vitamin D. The level in blood is regulated by the parathyroid gland.

Dietary sources of phosphorus

Phosphorus is present in most foods especially those rich in protein e.g. meat, poultry and fish, eggs dairy products and cereal products.

Deficiency of phosphorus

Phosphorus deficiency is unknown in humans but may affect people who consume large amounts of antacids especially those with aluminium hydroxide which interfere with phosphorus absorption. Premature infants may need more phosphorus than what is provided by breast milk.

Magnesium

The concentration of magnesium in the body is roughly 30mg; 60% of which is found in the bone. About a third of this is closely bound to phosphate and the remainder is on the surface of the bone from which it is readily mobilized to maintain normal blood and tissue levels. The blood contains about 1% of total body magnesium, a third of this is bound to the protein albumin, a third is free and a third is part of other compounds. It is absorbed in the small intestines with the help of a carrier. About half of the dietary intake is absorbed. Absorption is reduced by presence of calcium and alcohol phosphate, phytates and fats. Absorption is increased by dietary vitamin D and lactose. Excretion is regulated by the kidneys and reabsorption is facilitated.

 Functions of magnesium

a.   Within the cell, magnesium acts as a catalyst to many biological reactions especially those taking place in the mitochondrion of the cell. Magnesium is involved in cell reactions involving expenditure of energy from carbohydrate, fat and protein; and nucleic acid metabolism in those reactions related to the synthesis, degradation and stability of the genetic material including DNA.

b.   It is necessary for conduction of nerve impulses and for normal muscular contraction. In these roles, magnesium and calcium play antagonistic roles. Calcium stimulates whereas magnesium relaxes. A very high intake of magnesium could lead to coma and heart failure.

c.    Magnesium is also involved in bone formation together with phosphorus and calcium.

Dietary sources of magnesium

Dark green vegetables are the best sources of magnesium followed by legumes, sea food, nuts, cereals and dairy products. Water contains good amounts especially soft water; sea water has very high levels.

 Deficiency of magnesium

Magnesium deficiency is associated with starvation, persistent vomiting and trauma of surgery or diarrhoea.  A deficiency leads to weakness, confusion, growth failure in children. Severe deficiency leads to tetany (uncontrolled neuromuscular tremors which progress to convulsive seizures). Deficiency also leads to vasodilation (increase in diameter of blood vessels) and skin changes.

Sulphur

Sulphur represents only 0.25% of total body weight. It is concentrated in cytoplasm of the cell. High concentrations are found in the hair, skin and nails, hence the smell of sulphur dioxide when they are burned. The sulphur containing amino acids; cycteine and methionine are characteristic of the protein found in the tissues. Sulphur gets to the body through these sulphur-containing amino acids.


 

 Functions of magnesium

1.   Sulphur in combination with hydrogen plays an important role in metabolism.

2.   Involved in formation of blood clots.

3.   Involved in the transfer of energy.

4.   It is part of at least 3 vitamins; thiamin, pantothenic acid and biotin. These are part of coenzymes which activate several enzymes.

5.   Compounds containing sulphur act as detoxifying substances by combining toxic substances and converting them into harmless compounds that are excreted by the kidney.

6.   Sulphur is also necessary for collagen synthesis and the formation of many mucopolyssacharides.

 Fluorine

Fluorine is found mainly in the teeth and skeleton. Traces of fluorine in the teeth help to protect against decay. When consumed in early adulthood, it becomes part of the enamel, making it more resistant to the organic acids formed from foods that get stuck in between the teeth. Fluorine is also known to harden and strengthen the bone especially later in life and thus slow or inhibit the development of osteoporosis.

 Mode of action

Fluorine forms crystals of flourapatite which replaces crystals of hydroxypatite normally deposited during tooth formation. Flourapatite in the tooth enamel is more resistant to cavity forming acids. Absorption is known to occur in the mouth through the tooth surface. Intakes of 8ppm in adult life protects against osteoporosis (a condition where there is a reduction of the bone density due to erosion of calcium).

 Dietary sources of fluorine

·       The main source is water. If it contains 1ppm (part per million), this will be adequate.

·       Other sources include small fish when consumed whole.

·       Tea has a high content.

·       Foods grown in high fluoride water will have more than fresh foods.

Toxicity

Too much fluorine intake causes browning of teeth enamel and excessive amounts may cause dental flourosis in which the teeth become mottled.


 

 Micro elements

They are required in small amount by the body compared to the macro elements.

 Iron

Iron is found in every living cell. The total body content is about 5grams; varying slightly with age, sex, body size, nutritional status and general health. It is the only nutrient for which adult women have greater requirements than adult men.

 Absorption and distribution of iron

The body uses several mechanisms to regulate iron absorption because the body cannot easily eliminate excess iron once it is absorbed.5-10% of iron in food is absorbed in healthy people.10-20% of iron in food is absorbed in by people with iron deficiency. The extent of iron absorption depends on a variety of factors, the most important being the amount of current iron stores in the body.Iron in food occurs in several forms which differ in their absorption by the body. Iron that is part of haemoglobin and myoglobin molecules in animal flesh is about 40% of total iron present called heme iron and is readily absorbed more than twice as efficiently as simple elemental iron known as non-heme iron. Non-heme iron is provided from plant sources and elemental iron components of animal tissues.  It is less efficiently absorbed than heme iron. The difference in absorption of heme and non heme iron make animal flesh a rich source of dietary iron, considering both its iron content and the increased efficiency of absorption of the heme iron present.Consuming heme iron and non heme iron together increases non-heme iron absorption e.g. eating meat with vegetables and grain products.

 Factors affecting iron absorption

These include the following;

a.   Body needs

A normal person with normal haemoglobin levels absorbs only 2-10% of dietary non-heme iron and 23% of heme iron. Iron deficient individuals may absorb as much as a third of dietary iron from heme and a fifth from non-heme. Need for iron is increased with exercise because the production of red blood cells increases.


 

b.   Form of iron

Ferrous iron is absorbed better than the ferric iron. Organic acids such as vitamin C moderately increase non-heme absorption by adding an electron to ferric iron (Fe3+) yielding ferrous iron (Fe2+). Vitamin C then forms a complex called chelate with Fe 2+ thereby enhancing absorption. As such iron supplements should be taken together with vitamin C rich drinks such as fresh orange juice.

c.   Composition of meal

When meat is eaten with a vegetable and grain products, the absorption of non heme iron present is improved. A protein factor in meat, fish and poultry(MFP factor) facilitate non heme absorption. This factor appears to contain amino acids such as cysteine that bind iron to enhance absorption.

d.   Acid in the stomach

Acid also plays an important role in iron absorption by promoting the conversion of Fe3+ to Fe2+ and by solubilizing non heme iron. The decreased production of stomach acids experienced by many elderly people can lower their iron absorption and ultimately their body stores of iron.

 

 Dietary factors

Several dietary factors interfere with the body’s ability to absorb iron and these include;

Phytic acid and oxalic acid in vegetables which bind iron thus reducing its absorption.

Dietary fibre intakes above 35g/day bind iron and other trace elements.

Polyphenols such as tannins found in tea and related substances found in coffee also reduce iron absorption. People trying to rebuild iron stores are advised to reduce coffee and tea consumption, particularly meal times.

Calcium supplements in amounts greater than 300 mg/d can reduce iron absorption. Calcium supplements should be taken several hours before or after an iron rich meal.

 

Function of iron

1.   The major role of iron is to permit the transfer of oxygen and carbon dioxide from one tissue to another. Iron does this as part of haemoglobin in the blood and myoglobin in the tissues.

2.   Blood formation: Haemoglobin is an essential component of the red blood cells which are formed in the bone marrow when stimulated by erythropoietin, a hormone synthesized by the kidney. Erythropoietin is released in response to a decrease in oxygen occlusion in the blood, blood loss or binding of carbon dioxide to red blood cells. It targets the bone marrow to increase production of red blood cells.

3.   Other functions of iron: A small amounts of iron are incorporated into tissue enzymes. In a dietary deficiency, the level of these enzymes drops before the haemoglobin level drops.

Iron is also important in many other functions such as;

·       Catalyzing the conversion of beta carotene into vitamin A.

·       The synthesis of purines which form integral part of nucleic acid

·       The removal of lipids from the blood.

·       Collagen synthesis.

·       Antibody production needed for proper immune formation.

·       Detoxification of drugs in the liver.

 Iron needs

Iron needs differ for different age groups and sex. Pregnant women and adolescent girls need twice as much as iron compared to adult men. Iron needs are determined by need to replace losses through sweat, hair, nails, dead skin cells, masturbation or other forms of bleeding. Growth increases iron needs due to increased blood volume and tissue mass. The adult recommendations for iron are 10mg/d for men and 15mg/d for women to account for menstruation losses.

Pregnant women require 30mg/d daily. This takes care of the increased placenta blood volume and the foetal needs. New borns are born with enough iron stores to last for the first 4-6months until supplementary feeding is introduced. Human milk is a poor source of iron 15mg/d. Infants and children: Reserve of iron last 4–6months after delivery hence complementary foods introduced at 6 months need to be rich in iron.

 Dietary sources of iron

Liver is the richest source, lean meat, chicken, eggs, lean pork, dark green vegetables, legumes, nuts, molasses and cereals.

 Iron deficiency

Continued iron deficiency leads to nutritional anaemia caused by lack of dietary essentials involved in haemoglobin formation or poor absorption of these dietary essentials. Some types of anaemia have been reported to be caused by a lack of either dietary iron or high quality protein; lack of pyridoxine (vitamin B6), which catalyses the heme portion of the haemoglobin molecule, by a lack of vitamin C which influences the rate of iron absorption and the release of iron from transferring to the tissues; and by lack of vitamin E, which affects the stability of the red blood cell membrane. Copper is not part of the haemoglobin but aids in its synthesis by influencing the absorption of iron, its release from the liver, or its incorporation into haemoglobin.

Nutritional anaemia is characterised as both hypochromic and microcytic. Hypochromic suggests a lack of red pigment haemoglobin and microcytic indicates the presence of small red blood cells. When red blood cells synthesis is reduced, the amount of oxygen carried to the blood is decreased. There are many types of anaemia but the major type found in the world is iron deficiency anaemia. It is common in adolescent girls (growth and menstrual demands), young infants and women of child bearing ages.

 Iron toxicity

Excess intake may lead to hemosiderosis i.e. increased iron reserves due to excessive supplements. Hemochromatosis: A genetic disorder of iron metabolism characterized by increased absorption, saturation and deposition of iron in the liver tissues.

 Iodine

Iodine is present in the body in minute amounts, 15-23 mg and 75% of this is concentrated in the thyroid gland that uses it to synthesize the hormone, thyroxin. The remainder is in other tissues particularly in the salivary, mammary and gastric glands and in the kidney. It is present in food as iodide and other non elemental forms. Iodine was linked to the presence of an enlarged thyroid gland (goitre) during World War I.

 Functions of iodine

1.   Regulation of growth and development as part of thyroxin hormone. Thyroxin can stimulate metabolism by as much as 30%.

2.   Conversion of carotene to vitamin A.

3.   Synthesis of protein.

4.   When thyroxin levels are normal, absorption of carbohydrates from the intestines is more efficient.

5.   Synthesis of cholesterol.


 

 Recommended daily allowances:

For adults 150mg/day

 Dietary sources of iodine

These include: Salt water, fish, sea foods, iodized salt, molasses, dark green leafy vegetables especially spinach leaves.

 Deficiency of iodine

a.   Goitre

This is a condition characterised by swelling in the neck as a result of the enlargement of the thyroid gland. The gland enlarges as it attempts to compensate for lack of iodine essential to its major role in the synthesis of thyroxin. The pituitary gland hormone that stimulates the thyroid hormone production, thyroid stimulating hormone also stimulates the growth of the thyroid gland. Too much growth of the gland causes difficulties in breathing.

Goitre is also associated with the consumption of goitrogens (substance in foods such as the cabbage family and water that interfere with thyroid gland metabolism and thus may cause goitre if consumed in large amounts). Defects in the enzymes necessary for the synthesis and release of thyroxin can also lead to goitre. Goitre is common in areas where the soil is poor in iodine due to leaching. Goitre incidences are six times more in females than males with adolescent girls and pregnant women being more susceptible.

b.   Cretinism

If an expectant mother consumes iodine deficient diets during the early pregnancy, her infant may be born with a short stature (dwarfed) and with mental retardation. Maternal iodine needs take precedence over foetal needs. The retarded body growth is referred to as cretinism characterized by physical drawfness, mental retardation, thick-dry skin and enlarged protruding stomach.

c.   Myxedema

This is a condition seen in adults who have had symptoms of hypothyroidism throughout their development and growth period. It is characterized by coarse sparse hair, dry, yellowish hair, poor tolerance to cold and low husky voice.

d.   Hyperthyroidism

This is where basal metabolic rate is accelerated by as much as 100% above normal and is associated with over activity of the thyroid gland and experience nervousness, weight loss, increased appetite, intolerance to heat, tremors and protruding eyeballs.


 

 Zinc

Zinc is present in all body tissues and fluids .The total human body zinc content has been estimates to be 30 mmol (2grams). Skeleton muscle accounts for approximately 60% of the total body content and bone mass, with a zinc concentration of 1.5-3.0mol/g (100-200g/g) for approximately 30%. The concentration of zinc in lean body mass is approximately 0.46 mol/g (30(g/1). Plasma zinc has a rapid turn over and represent only about 0.1% of total body zinc content. This level appears to be under close homeostatic control. High concentrations of zinc are found in the choroids of the eye (4.2 mol/g or 274 g/g) and in prostastic fluids (4.6-7.7 mmol/l or 300-500mg/l).  Zinc is lost through faeces, urine and shed tissues. High tissue losses are from the skin, the mucosal cells, menstrual fluids and semen.

 Absorption of zinc

Like iron absorption, zinc absorption is influenced by the types of food ingested. More is absorbed when animal protein is consumed. Fibre and phytates inhibit zinc absorption.

 Functions of zinc

Over 300 enzymes require zinc as a co-factor for optimal activity. Adequate zinc is necessary to support many bodily functions such as;

a.   It is involved in nucleic acid synthesis and function (some factors that control expression of genes contains zinc-rich regions that bind DNA).

b.   It is involved in protein metabolism, growth and wound healing.

c.    Immune function.

d.   Development of sexual organs and bone.

e.    Involved in storage, release and function of insulin.

f.     Cell membrane structure and function.

Recommended daily requirements for zinc

The adult recommended daily requirement is 15 mg/day for men and 12 mg/day for women.

Dietary sources of zinc

In general, protein-rich diets are also rich in zinc. Lean meats especially beef and other red meats, eggs and shell fish are the best sources. Cereals and legumes contain significant amounts of zinc but contain phytic acid and other substances that can interfere with intestinal absorption of zinc.


 

 Zinc deficiency

Zinc deficiency is associated with;

·       Severe growth retardation and arrested sexual maturation.

·       Altered taste acuity.

·       Impaired immune function.

·       Slow wound healing.

·       Impaired central nervous system function.

Selenium

Selenium is deposited in all body tissues except fat. The highest concentrations occur in the liver, kidney, heart and spleen. Serum levels are about 0.22 µg/dl. Most selenium in foods is bound to derivatives of the amino acids; methionine and cysteine. Because these substances are readily absorbed, bioavailability of selenium is considerably higher than that of zinc and iron. About 50%-100 % of dietary selenium intake is absorbed. In addition, since no physiological mechanism appears to control selenium absorption, selenium has a definite potential for toxicity. Most selenium is excreted via the urine and faeces.

 Functions of selenium

a.   It functions as an integral component of an anti oxidant enzyme; glutathione peroxidase that protects cells and lipid membranes against oxidative damage.

b.   It also acts as a structural component, incorporated into the protein matrix of the teeth.

 Recommended daily allowances for selenium

The recommended daily allowance estimated to be safe and adequate for an adult is about 50-200 µg/day.

Dietary sources of selenium

Food sources vary with the selenium soil content. Good sources include sea food, legumes, whole grains, low fat meats and dairy products with smaller amounts in vegetables.

Selenium deficiency

The signs and symptoms of selenium deficiency include pain, muscle wasting and cardiomyopathy, a form of heart disease.

 Selenium toxicity

High doses (a milligram or above daily is toxic. Selenium toxicity causes vomiting, diarrhoea, loss of hair and nervous system.


 

 Other minerals

In addition to macro and micro elements discussed above, other mineral elements are present in the body in variable amounts and are essential in human metabolism. In the human body, they are almost always bound to organic compounds rather than being free in organic elements. They are copper, manganese, molybdenum, chromium, nickel, vanadium, silicon, tin and cobalt. Copper is a component of oxidative enzymes; cytochrome c oxidase which is active at terminal end of the mitochondrial electron transport chain.  Molybdenum acts as part of several metalloenzymes that are involved in oxidation-reduction reactions and may be involved with detoxification process in the liver. It appears to be particularly important in the early stages of brain development.  Chromium participates in carbohydrate and lipid metabolism. The only known function of cobalt is as part of vitamin B12.

 Summary

·       Minerals are inorganic substances that are widely distributed in nature. They build body tissues, active, regulate and control metabolic processes, transmit neorologic messages.

·       They are classified as; macro elements that are required in relatively large quantities, which make up 60-80% of all inorganic material in the body and the micro elements are required in quantities as small as microgram, which make up less than 1% of the body’s inorganic material.

·       Many minerals are vital for sustaining life. For humans, animal products are typically the best sources of most minerals.

·       Calcium forms a vital part of the bone structure and is also very important cation (Ca2+) in blood clotting, muscle contraction and nerve-impulse transmission. Calcium absorption is enhanced by stomach acid and many other factors.Dairy products are important calcium sources.

·       Phosphorus aids the function of many enzymes and forms part of ATP molecules, numerous metabolites and phospholipids in cell membranes. It is efficiently absorbed, deficiencies are rare. Good food sources are dairy products, bakery products and meats.

·       Magnesium is the only mineral found mostly in plants where it functions in chlorophyll. In humans, it is important for nerve, lung and heart function, as a co-factor for many co-enzymes. Good sources include dark green vegetables, legumes sea food, nuts, cereals and dairy products. Sulphur is incorporated into certain vitamins and amino acids.

·       Fluorine is found mainly in teeth and skeleton and the main sources is water, small fish and some amounts are also found in tea.

·       Iron is found in every cell in the body. It is a critical component of haemoglobin, myoglobin and cytochromes. It aids in the transfer of oxygen and carbon dioxide from one tissue to another. It is also involved in blood formation, conversion of ß-carotene to vitamin A, synthesis of purines, collagen formation, and removal of lipids in blood, antibody production and detoxification of drugs in the liver.

·       Iodine is concentrated in the thyroid gland that uses it to synthesise thyroxin hormone that regulates growth and development. A lack of dietary iodide results in development of goitre and other metabolic problems including stunted growth and mental retardation.

·       Zinc functions as a co-factor for over 300 enzymes that are important for growth, development, immune function, wound healing and taste sensation. The most understood role of selenium is as a co-factor for glutathione peroxidase, whose action reduces oxidative damage of cells.

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